pcfj601 (Addgene inc)

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Addgene inc pcfj601
Pcfj601, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 39 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/plasmid+pcfj601/pCFJ601+-+Peft-3+Mos1+transposase+(Plasmid+%2334874)/pm41673492-246-52-55
Average 93 stars, based on 39 article reviews

pcfj601 - by Bioz Stars, 2026-09

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Article Title: Multiple Histone Methyl-Lysine Readers Ensure Robust Development and Germline Immortality in Caenorhabditis elegans
Article Snippet: Plasmids were microinjected along with the transposase vector pCFJ601 ( Peft-3:Mos1 , #34874; Addgene plasmid) and co-injection markers as described ( Frøkjær-Jensen et al. 2014 ).

Article Title: Neuronal exosomes transport an miRISC cargo to preserve stem cell integrity during energy stress.
Article Snippet: To generate a kinase dead variant of SID-3, aaa (lysine) was mutated into gca (alanine) in the MR1963 daf-2; aak(0); tbc-7(rr166) strain using CRISPR-Cas9 with sgRNA sequence primer 37 and a repair template/ultramer DNA oligonucleotide (Integrated DNA Technologies) containing the desired K139A mutation.50 The sequence of the repair template: 50-CGTGGACACAGAGCAATGG GACGCATGTGAATGTCGCTGTggcAATcCTgCGCGAtATcTCcCCtAATATTATGGATGATTTGAGAgtgagttctacttgtatg-30 Transgenic animals were generated by injecting transgenes (15 ng mL 1 each) mixed with a co-injection marker pRF-4 (120 ng mL 1) (Addgene) expressing a dominant negative variant of the rol-6 gene and an empty vector pSK (up to 200 ng mL 1 of DNA) (Addgene) using standard methods.64 To create transgenic animals with germline his-15::gfp expression, EG669962 (ttTi5605 II; unc-119(ed3) III; oxEx1578) animals were injected with a target transgene (50 ng mL 1) with plasmid pJL43.1 (50 ng mL 1) (Addgene) that expresses Mos1 transposase under a germline promoter and plasmid pMR910 (20 ng mL 1) that expresses GFP under a pharyngeal promoter using standard methods.64 To create transgenic animals with neuronal HA::GFP::RAB-7 or mKate::3xFLAG::ALG-1 expression, EG6703 (unc-119(ed3) III; cxTi10816 IV; oxEX1582) animals were injected with a target transgene (50 ng mL 1) with plasmid pCFJ601 (50 ng mL 1) (Addgene) that expresses Mos1 transposase under a ubiquitous promoter and plasmid pMR910 (20 ng mL 1) that expresses GFP under a pharyngeal promoter using standard methods.64

Polymerase Chain Reaction:

Article Title: Heritable genome editing in C. elegans via a CRISPR-Cas9 system
Article Snippet: .. The promoter region from the eft-3 gene and 3′ UTR from the gene tbb-2 were PCR-amplified from plasmid pCFJ601 (obtained from Addgene through the kind gift of E. Jorgensen and C. Frokjaer-Jensen) using the primers pUC57 EcoRI Peft-3 F / Peft-3 cas9 start R and tbb-2 UTR F / tbb-2 UTR pUC57 R , respectively. ..

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